Scientific and technical platforms supporting cancer research

The Genomics Unit provides comprehensive support for genetic material analysis, offering services such as DNA sequencing, gene expression profiling, and genotyping.

The facility is equipped to analyze RNA and DNA samples using advanced technologies to ensure high-quality and reproducible results.

The Unit offers support to both CIC and external users, managing the complete analytical process from the experimental sample to technical advice, experimental design and quality control.

• Complete microarray and sequencing services
• Support for CIC and external users
• Certified quality and safety management systems

The Proteomics Unit offers access to cutting-edge platforms for the comprehensive study and identification of proteins and peptides.

Its services cover protein identification and quantification, comparative proteomics, analysis of post-translational modifications and investigation of protein–protein interactions.

The Unit provides advice and support during experimental design, sample preparation, nano-LC separation, tandem mass spectrometry analysis and comprehensive data analysis.

For quantitative proteomics, the Unit offers both label-free approaches and isotopic labeling methods, including SILAC.

• End-to-end proteomics support
• Mass spectrometry-based protein identification and quantification
• Advanced bioinformatics interpretation
• Academic and industry collaborations

The Advanced Cellular Analyses Unit provides researchers with cutting-edge imaging and cytometry technologies for cellular and molecular studies.

Microscopy services cover widefield, confocal laser scanning, nanoscopy and mesoscopy, supporting both fixed and live-cell imaging.

Advanced applications include super-resolution imaging such as STED, TIRF-M and PALM/STORM, as well as molecular interaction studies by FLIM-FRET and crossN&B.

The Unit also offers high-performance flow cytometry services, including multiparametric cell analysis and fluorescence-activated cell sorting.

The Unit is open to CIC research groups and external institutions, offering technical support, training and consultation.

• High-resolution imaging technologies
• Super-resolution microscopy
• Advanced flow cytometry and cell sorting
• Training and consultation for internal and external users

The Pathology Unit specializes in the histological, immunohistochemical and molecular analysis of human, animal and plant tissue samples.

The Unit offers services tailored to researchers’ needs, including routine tissue staining, immunohistochemistry, multiplexing, tissue microarrays and laser microdissection microscopy.

Histopathology services include tissue processing, paraffin embedding, sectioning and hematoxylin-eosin staining for morphological evaluation.

Laser microdissection technology enables the isolation of specific cells from complex tissues for molecular characterization.

The Unit contributes to education and training, student mentorship programs and collaborative research initiatives.

• Histology, immunohistochemistry and molecular tissue analysis
• Biobanking coordination through BEOCyL
• NUCLEUS-associated research support service
• Quality and occupational safety certifications

The Bioinformatics Unit works at CIC with the help and support of the Bioinformatics and Functional Genomics Research Group.

The Unit provides specialized computational analysis services to support research in genomics, transcriptomics, proteomics and other omics disciplines.

The team assists scientists in data processing, interpretation and integration, applying advanced bioinformatics tools to extract meaningful insights from complex biological datasets.

The Unit has extensive expertise in high-throughput sequencing data analysis, including RNA-Seq, whole-genome sequencing and custom panel analysis.

Since its establishment in 2008, the Unit has analysed over 5,000 datasets from diverse experimental platforms, supporting both academic and industry projects.

• Over 5,000 datasets analysed since 2008
• Support for academic and industry projects
• Part of the CSIC-INB/ELIXIR Spain network

The Mouse Model Experimentation Unit (UEMM) offers comprehensive support to CIC users conducting research with mouse models.

During this period, the Unit was staffed by two full-time technicians with over 15 and 5 years of experience, respectively, as well as two technicians from the Youth Guarantee Program of the Junta de Castilla y León.

The Unit uses a weekly task request system, allowing users to specify required tasks in advance.

The UEMM maintains the mouse colony management software Raton Manager, which houses detailed information on all mouse colonies.

For genotyping services, the Unit provides a photograph of the gel and a detailed report including mouse number, sex, weaning cage and parentage.

• Comprehensive support for mouse model research
• Weekly task request system
• Mouse colony management through Raton Manager
• Genotyping reports with gel photograph and mouse metadata